Roy Goodacre at the 2020 Breath Biopsy Conference
Study Design session (35 min) - Metabolomics by numbers: Lessons from large-scale phenotyping
The 2021 Breath Biopsy Conference is scheduled for 12th & 13th October. Click the button to express interest in the next Breath Biopsy Conference:
Talk Abstract:
Metabolomics is a growing discipline that allows the analysis of the thousands of structural different small molecules found within a biological system. These metabolites can be measured using a variety of different analytical approaches and we have developed gas chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) for this purpose1. I shall provide a brief overview of serum-based metabolomics and lessons learnt from of our large-scale human serum metabolome project where we profiled 1200 healthy individuals2. Using these protocols, we then went on to profile another ~1200 ageing individuals and identified key metabolic dysregulation which were drivers behind human frailty, which were validated in a further ~760 ageing individuals3.
1. Dunn, W.B. et al (2011) Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nature Protocols 6, 1060-1083.
2. Dunn, W.B. et al (2015) Molecular phenotyping of a UK population: defining the human serum metabolome. Metabolomics 11, 9-26.
4. Rattray, N.J.W. et al (2019) Metabolic dysregulation in vitamin E and carnitine shuttle energy mechanisms identified as drivers behind human frailty. Nature Communications 10: 5027.
Speaker Biography:
Roy Goodacre is Professor of Biological Chemistry at the University of Liverpool. His research interests are broadly within analytical biotechnology and systems, and synthetic biology. He has helped establish mass spectrometry-based metabolomics for long-term studies and employed metabolomics for clinical and plant studies, as well as for understanding microbial systems. He has also developed a variety of different Raman spectroscopy approaches for bioanalysis with a particular focus on metabolite quantification and chemical image analysis.
He helped establish the Metabolomics Society, is Editor-in-Chief of the journal Metabolomics and sits on the Editorial Advisory Boards of four other journals.
The 2021 Breath Biopsy Conference is scheduled for 12th & 13th October. Click the button to express interest in the next Breath Biopsy Conference: