Yuta Matsuoka at BBCon 2024
Volatile oxidized lipids generated via metal-dependent lipid peroxidation of ω-6 PUFAs are breath biomarkers for monitoring ferroptosis in vivo
00:00 Introductions
00:48 Presentation: ‘Volatile Oxidized Lipids Generated via Metal-Dependent Lipid Peroxidation of w-6 PUFAs are Breath Biomarkers for Monitoring Ferroptosis in Vivo’
20:10 Q&A
Talk Abstract:
Volatile organic compounds resulting from metabolic processes are released as biogas, and potentially can serve as noninvasive biomarkers of cellular metabolic health. During ferroptosis, a form of cell death caused by lipid peroxidation (LPO), oxidized lipid fragments can be released as biogas. We identified ω-6 PUFA-derived volatile oxidized lipids (VOLs) produced and released by cells undergoing metal-dependent LPO. Among several identified VOLs, two VOLs were validated as ferroptosis markers in in vitro cultured cells, in vivo animal models, and in human breath. We find these VOLs specifically produced in the liver during ferroptosis, in model mice of metabolic dysfunction-associated steatohepatitis (MASH) and in the exhaled air from patients with MASH. Interestingly, in the MASH and Liver cirrhosis groups, VOL marker showed a moderate correlation (r > 0.6) with fibrosis marker mac-2-binding protein glycosylation isomer (M2BPGi), and liver function marker albumin-bilirubin (ALBI) score (r > 0.6). Our identification of specific VOLs released upon metal-dependent LPO, highlights their potential utilization as valuable markers for cells undergoing ferroptosis in vivo, which can be apply non-invasively to monitoring cellular health in humans.
Yuta has also recently published a pre-print of the study which he discusses this talk.
Speaker Biography:
Yuta Matsuoka is the assistant professor from Kyoto University Graduate School of Medicine. Throughout his career, he has advanced research concerning the development and application of lipid peroxidation analysis techniques (Anal.Chem. 2020, Nat. Commun. 2021). Recently, he has been expanding his research into the development of breath biopsy technology focusing on “volatile oxidized lipids” and “ferroptosis”.
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